Modeling population dynamics in a microbial consortium under control of a synthetic pheromone-mediated communication system.

ABSTRACT: Microbial consortia can be used to catalyze complex biotransformations. Tools to control the behavior of these consortia in a technical environment are currently lacking. In the present study, a synthetic biology approach was used to build a model consortium of two Saccharomyces cerevisiae strains where growth and expression of the fluorescent marker protein EGFP by the receiver strain is controlled by the concentration of α-factor pheromone, which is produced by the emitter strain. We have developed a quantitative experimental and theoretical framework to describe population dynamics in the model consortium. We measured biomass growth and metabolite production in controlled bioreactor experiments, and used flow cytometry to monitor changes of the subpopulations and protein expression unde

SUBMITTER: Hoffmann A 

PROVIDER: S-EPMC6999395 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature