Phospho-Profiling Linking Biology and Clinics in Pediatric Acute Myeloid Leukemia.

ABSTRACT: Aberrant activation of key signaling-molecules is a hallmark of acute myeloid leukemia (AML) and may have prognostic and therapeutic implications. AML summarizes several disease entities with a variety of genetic subtypes. A comprehensive model spanning from signal activation patterns in major genetic subtypes of pediatric AML (pedAML) to outcome prediction and pre-clinical response to signaling inhibitors has not yet been provided. We established a high-throughput flow-cytometry based method to assess activation of hallmark phospho-proteins (phospho-flow) in 166 bone-marrow derived pedAML samples under basal and cytokine stimulated conditions. We correlated levels of activated phospho-proteins at diagnosis with relapse incidence in intermediate (IR) and high risk (HR) subtypes. In paralle

SUBMITTER: Schumich A 

PROVIDER: S-EPMC7000467 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature