MiR-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting KLF5

ABSTRACT:

Background/aim

Diabetic vascular smooth muscle cells (VSMCs) are characterized by increased proliferation and migration. Small noncoding microRNAs (miRNAs) have been considered critical modulators of the VSMC phenotypic switch after an environmental stimulus. However, microRNA in high glucose-induced proinflammation and its atherogenic effect is still ambiguous.

Materials and methods

The technique of qRT-PCR was used to examine the expression of miR-9 in VSMCs. The downstream signaling protein relative to miR-9 regulation, Krüppel-like factor 5, and some marker genes of contractile VSMCs were analyzed by western blotting and qRT-PCR. Luciferase reporter assay was used to detect the expression of KLF5, which is regulated by miR-9. To examine

SUBMITTER: Lu X 

PROVIDER: S-EPMC7018344 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature