The buffy coat method: a tool for detection of blood parasites without staining procedures.

ABSTRACT: BACKGROUND:Blood parasites belonging to the Apicomplexa, Trypanosomatidae and Filarioidea are widespread in birds and have been studied extensively. Microscopical examination (ME) of stained blood films remains the gold standard method for the detection of these infections in birds, particularly because co-infections predominate in wildlife. None of the available molecular tools can detect all co-infections at the same time, but ME provides opportunities for this to be achieved. However, fixation, drying and staining of blood films as well as their ME are relatively time-consuming. This limits the detection of infected hosts during fieldwork when captured animals should be released soon after sampling. It is an obstacle for quick selection of donor hosts for parasite experimental, histolog

SUBMITTER: Chagas CRF 

PROVIDER: S-EPMC7045512 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature