Comparison of osteogenic and dentinogenic potentials of mice incisor and molar pulps in vitro.

ABSTRACT:

Objective

In the present study, we compared the in vitro osteogenic and dentinogenic potential of pulp cells from incisors and molars.

Design

Primary pulp cultures were established from DSPP-Cerulean/DMP1-Cherry and BSP-GFPtpz reporter mouse lines and processed for various assays.

Results

Our results showed marked differences in dentinogenic and osteogenic potentials of primary cultures from unerupted molars and incisors isolated from 5 to 7 days old pups. While primary cultures from both incisors and molars differentiated into odontoblasts and osteoblasts, cultures from molars differentiated into more DSPP-Cerulean + cells (∼5.5 %) compared to incisor cultures (∼0.7 %) at Day 14 and appear to be more committed to the odontogenic lineage. On the other hand, cultures f

SUBMITTER: Vijaykumar A 

PROVIDER: S-EPMC7050286 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature