Identification of novel differentially expressed genes in retinas of STZ-induced long-term diabetic rats through RNA sequencing.

ABSTRACT:

Background

The aim of this research was to investigate the retinal transcriptome changes in long-term streptozotocin (STZ)-induced rats' retinas using RNA sequencing (RNA-seq), to explore the molecular mechanisms of diabetic retinopathy (DR), and to identify novel targets for the treatment of DR by comparing the gene expression profile we obtained.

Methods

In this study, 6 healthy male SD rats were randomly divided into wild-type (WT) group and streptozotocin (STZ)-induced group, 3 rats each group. After 6 months, 3 normal retina samples and 3 DM retina samples (2 retinas from the same rat were considered as 1 sample) were tested and differentially expressed genes (DEGs) were measured by RNA-seq technology. Then, we did Gene Ontology (GO) enrichment analysis and KEGG (Kyoto

SUBMITTER: Xing X 

PROVIDER: S-EPMC7057111 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature