Changes in Microbiota Profiles After Prolonged Frozen Storage of Stool Suspensions.

ABSTRACT: Introduction: Fecal microbiota transplantation (FMT) is recommended as safe and effective treatment for recurrent Clostridioides difficile infections. Freezing the FMT preparation simplifies the process, allowing a single stool sample to be used for multiple receivers and over an extended period of time. We aimed to assess the effect of long-term frozen storage on bacterial taxonomic profiles of a stool suspension prepared for FMT. Methods: DNA was extracted from a stool suspension before freezing and sequentially during the 18-month storage period at -80°C. Two different protocols were used for DNA extraction. The first relied on a classical mechanical and chemical cell disruption to extract both intra- and extracellular DNA; the second included specific pre-treatment

SUBMITTER: Dorsaz S 

PROVIDER: S-EPMC7058979 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature