Comparison of stool versus rectal swab samples and storage conditions on bacterial community profiles.

ABSTRACT:

Background

Sample collection for gut microbiota analysis from in-patients can be challenging. Collection method and storage conditions are potential sources of variability. In this study, we compared the bacterial microbiota from stool stored under different conditions, as well as stool and swab samples, to assess differences due to sample storage conditions and collection method.

Methods

Using bacterial 16S rRNA gene sequence analysis, we compared the microbiota profiles of stool samples stored and collected under various conditions. Stool samples (2 liquid, 1 formed) from three different patients at two hospitals were each evaluated under the following conditions: immediately frozen at -80°C, stored at 4°C for 12-48 hours before freezing at -80°C and stored at -20°C with 1

SUBMITTER: Bassis CM 

PROVIDER: S-EPMC5374586 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature