Characterization of an l-Ascorbate Catabolic Pathway with Unprecedented Enzymatic Transformations.

ABSTRACT: l-Ascorbate (vitamin C) is ubiquitous in both our diet and the environment. Here we report that Ralstonia eutropha H16 (Cupriavidus necator ATCC 17699) uses l-ascorbate as sole carbon source via a novel catabolic pathway. RNaseq identified eight candidate catabolic genes, sequence similarity networks, and genome neighborhood networks guided predictions for function of the encoded proteins, and the predictions were confirmed by in vitro assays and in vivo growth phenotypes of gene deletion mutants. l-Ascorbate, a lactone, is oxidized and ring-opened by enzymes in the cytochrome b₅₆₁ and gluconolactonase families, respectively, to form 2,3-diketo-l-gulonate. A protein predicted to have a WD40-like fold catalyzes an unprecedented benzilic acid rea

SUBMITTER: Stack TMM 

PROVIDER: S-EPMC7059403 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature