Dataset Information
Transcriptome-wide piRNA profiling in human brains for aging genetic factors.
Ontology highlight
ABSTRACT: Objective
Piwi-interacting RNAs (piRNAs) represent a molecular feature shared by all nonaging biological systems, including the germline and somatic cancer stem cells, which display an indefinite renewal capacity and lifespan-stable genomic integrity and are potentially immortal. Here, we tested the hypothesis that piRNA is a critical genetic determinant of aging in humans.Methods
Expression of transcriptome-wide piRNAs (n=24k) was profiled in the human prefrontal cortex of 12 subjects (84.9±9.5, range 68-100, years of age) using microarray technology. We examined the correlation between these piRNAs' expression levels and age, adjusting for covariates including disease status.Results
A total of 9,453 piRNAs were detected in brain. Including seven intergenic and three intronic piRNAs, ten piRNAs were significantly associated with age after correction for multiple testing (|r|=0.9; 1.9×10-5≤p≤9.9×10-5).Conclusion
We conclude that piRNAs might play a potential role in determining the years of survival of humans. The underlying mechanisms might involve the suppression of transposable elements (TEs) and expression regulation of aging-associated genes.
SUBMITTER: Mao Q
PROVIDER: S-EPMC7059831 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Transcriptome-wide piRNA profiling in human brains for aging genetic factors.
Jacobs journal of genetics 20190820 1
<h4>Objective</h4>Piwi-interacting RNAs (piRNAs) represent a molecular feature shared by all nonaging biological systems, including the germline and somatic cancer stem cells, which display an indefinite renewal capacity and lifespan-stable genomic integrity and are potentially immortal. Here, we tested the hypothesis that piRNA is a critical genetic determinant of aging in humans.<h4>Methods</h4>Expression of transcriptome-wide piRNAs (n=24k) was profiled in the human prefrontal cortex of 12 su ...[more]