Transcriptome-wide piRNA profiling in human brains for aging genetic factors.

ABSTRACT:

Objective

Piwi-interacting RNAs (piRNAs) represent a molecular feature shared by all nonaging biological systems, including the germline and somatic cancer stem cells, which display an indefinite renewal capacity and lifespan-stable genomic integrity and are potentially immortal. Here, we tested the hypothesis that piRNA is a critical genetic determinant of aging in humans.

Methods

Expression of transcriptome-wide piRNAs (n=24k) was profiled in the human prefrontal cortex of 12 subjects (84.9±9.5, range 68-100, years of age) using microarray technology. We examined the correlation between these piRNAs' expression levels and age, adjusting for covariates including disease status.

Results

A total of 9,453 piRNAs were detected in brain. Including seven intergenic and thr

SUBMITTER: Mao Q 

PROVIDER: S-EPMC7059831 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature