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Structure of SWI/SNF chromatin remodeller RSC bound to a nucleosome.


ABSTRACT: Chromatin-remodelling complexes of the SWI/SNF family function in the formation of nucleosome-depleted, transcriptionally active promoter regions (NDRs)1,2. In the yeast Saccharomyces cerevisiae, the essential SWI/SNF complex RSC3 contains 16 subunits, including the ATP-dependent DNA translocase Sth14,5. RSC removes nucleosomes from promoter regions6,7 and positions the specialized +1 and -1 nucleosomes that flank NDRs8,9. Here we present the cryo-electron microscopy structure of RSC in complex with a nucleosome substrate. The structure reveals that RSC forms five protein modules and suggests key features of the remodelling mechanism. The body module serves as a scaffold for the four flexible modules that we call DNA-interacting, ATPase, arm and actin-related protein (ARP) modules. The DNA-interacting module binds extra-nucleosomal DNA and is involved in the recognition of promoter DNA elements8,10,11 that influence RSC functionality12. The ATPase and arm modules sandwich the nucleosome disc with the Snf2 ATP-coupling (SnAC) domain and the finger helix, respectively. The translocase motor of the ATPase module engages with the edge of the nucleosome at superhelical location +2. The mobile ARP module may modulate translocase-nucleosome interactions to regulate RSC activity5. The RSC-nucleosome structure provides a basis for understanding NDR formation and the structure and function of human SWI/SNF complexes that are frequently mutated in cancer13.

SUBMITTER: Wagner FR 

PROVIDER: S-EPMC7093204 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Structure of SWI/SNF chromatin remodeller RSC bound to a nucleosome.

Wagner Felix R FR   Dienemann Christian C   Wang Haibo H   Stützer Alexandra A   Tegunov Dimitry D   Urlaub Henning H   Cramer Patrick P  

Nature 20200311 7799


Chromatin-remodelling complexes of the SWI/SNF family function in the formation of nucleosome-depleted, transcriptionally active promoter regions (NDRs)<sup>1,2</sup>. In the yeast Saccharomyces cerevisiae, the essential SWI/SNF complex RSC<sup>3</sup> contains 16 subunits, including the ATP-dependent DNA translocase Sth1<sup>4,5</sup>. RSC removes nucleosomes from promoter regions<sup>6,7</sup> and positions the specialized +1 and -1 nucleosomes that flank NDRs<sup>8,9</sup>. Here we present th  ...[more]

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