Structure of SWI/SNF chromatin remodeller RSC bound to a nucleosome.

ABSTRACT: Chromatin-remodelling complexes of the SWI/SNF family function in the formation of nucleosome-depleted, transcriptionally active promoter regions (NDRs)^1,2. In the yeast Saccharomyces cerevisiae, the essential SWI/SNF complex RSC³ contains 16 subunits, including the ATP-dependent DNA translocase Sth1^4,5. RSC removes nucleosomes from promoter regions^6,7 and positions the specialized +1 and -1 nucleosomes that flank NDRs^8,9. Here we present the cryo-electron microscopy structure of RSC in complex with a nucleosome substrate. The structure reveals that RSC forms five protein modules and suggests key features of the remodelling mechanism. The body module serves as a scaffold for the four flexible modules that we call DNA-interacting, ATPas

SUBMITTER: Wagner FR 

PROVIDER: S-EPMC7093204 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature