Dataset Information

Calcitonin Receptor Neurons in the Mouse Nucleus Tractus Solitarius Control Energy Balance via the Non-aversive Suppression of Feeding.

ABSTRACT: To understand hindbrain pathways involved in the control of food intake, we examined roles for calcitonin receptor (CALCR)-containing neurons in the NTS. Ablation of NTS Calcr abrogated the long-term suppression of food intake, but not aversive responses, by CALCR agonists. Similarly, activating Calcr^NTS neurons decreased food intake and body weight but (unlike neighboring Cck^NTS cells) failed to promote aversion, revealing that Calcr^NTS neurons mediate a non-aversive suppression of food intake. While both Calcr^NTS and Cck^NTS neurons decreased feeding via projections to the PBN, Cck^NTS cells activated aversive CGRP^PBN cells while Calcr^NTS cells activated distinct non-CGRP PBN cells. Hence, Calcr^NTS cells suppress feeding via non-aversive, non-CGRP PBN targets. Additionally, silencing Calcr^NTS cells blunted food intake suppression by gut peptides and nutrients, increasing food intake and promoting obesity. Hence, Calcr^NTS neurons define a hindbrain system that participates in physiological energy balance and suppresses food intake without activating aversive systems.

SUBMITTER: Cheng W

PROVIDER: S-EPMC7104375 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Calcitonin Receptor Neurons in the Mouse Nucleus Tractus Solitarius Control Energy Balance via the Non-aversive Suppression of Feeding.

Cheng Wenwen W Gonzalez Ian I Pan Warren W Tsang Anthony H AH Adams Jessica J Ndoka Ermelinda E Gordian Desiree D Khoury Basma B Roelofs Karen K Evers Simon S SS MacKinnon Andrew A Wu Shuangcheng S Frikke-Schmidt Henriette H Flak Jonathan N JN Trevaskis James L JL Rhodes Christopher J CJ Fukada So-Ichiro SI Seeley Randy J RJ Sandoval Darleen A DA Olson David P DP Blouet Clemence C Myers Martin G MG

Cell metabolism 20200116 2

To understand hindbrain pathways involved in the control of food intake, we examined roles for calcitonin receptor (CALCR)-containing neurons in the NTS. Ablation of NTS Calcr abrogated the long-term suppression of food intake, but not aversive responses, by CALCR agonists. Similarly, activating CalcrNTS neurons decreased food intake and body weight but (unlike neighboring CckNTS cells) failed to promote aversion, revealing that CalcrNTS neurons mediate a non-ave ...[more]

PMID: 31955990

Similar Datasets

Project description:The nucleus tractus solitarius (NTS) receives subdiaphragmatic visceral sensory information via vagal A- or C-fibers. We have recently shown that, in contrast to cardiovascular NTS medialis neurons, which respond to either purinergic or vanilloid agonists, the majority of esophageal NTS centralis (cNTS) neurons respond to vanilloid agonists, whereas a smaller subset responds to both vanilloid and purinerigic agonists. The present study aimed to further investigate the neurochemical and synaptic characteristics of cNTS neurons using whole cell patch-clamp, single cell RT-PCR and immunohistochemistry. Excitatory postsynaptic currents (EPSCs) were evoked in cNTS by tractus solitarius stimulation, and in 19 of 64 neurons perfusion with the purinergic agonist αβ-methylene ATP (αβMeATP) increased the evoked EPSC amplitude significantly. Furthermore, neurons with αβMeATP-responsive synaptic inputs had different probabilities of release compared with nonresponsive neurons. Single cell RT-PCR revealed that 8 of 13 αβMeATP-responsive neurons expressed metabotropic glutamate receptor 8 (mGluR8) mRNA, which our previous studies have suggested is a marker of glutamatergic neurons, whereas only 3 of 13 expressed glutamic acid dehydroxylase, a marker of GABAergic neurons. A significantly lower proportion of αβMeATP-nonresponsive neurons expressed mGluR8 (2 of 30 neurons), whereas a greater proportion expressed glutamic acid dehydroxylase (12 of 30 neurons). Esophageal distension significantly increased the number of colocalized mGluR8- and c-Fos-immunoreactive neurons in the cNTS from 8.0 ± 4% to 20 ± 2.5%. These data indicate that cNTS comprises distinct neuronal subpopulations that can be distinguished based on their responses to purinergic agonists and that these subpopulations have distinct neurochemical and synaptic characteristics, suggesting that integration of sensory inputs from the esophagus relies on a discrete organization of synapses between vagal afferent fibers and cNTS neurons.

Dataset Information

Calcitonin Receptor Neurons in the Mouse Nucleus Tractus Solitarius Control Energy Balance via the Non-aversive Suppression of Feeding.

Publications

Calcitonin Receptor Neurons in the Mouse Nucleus Tractus Solitarius Control Energy Balance via the Non-aversive Suppression of Feeding.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets