Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures.

ABSTRACT: Genome editing technologies have transformed our ability to engineer desired genomic changes within living systems. However, detecting precise genomic modifications often requires sophisticated, expensive, and time-consuming experimental approaches. Here, we describe DTECT (Dinucleotide signaTurE CapTure), a rapid and versatile detection method that relies on the capture of targeted dinucleotide signatures resulting from the digestion of genomic DNA amplicons by the type IIS restriction enzyme AcuI. DTECT enables the accurate quantification of marker-free precision genome editing events introduced by CRISPR-dependent homology-directed repair, base editing, or prime editing in various biological systems, such as mammalian cell lines, organoids, and tissues. Furthermore, DTECT allows the ide

SUBMITTER: Billon P 

PROVIDER: S-EPMC7108696 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature