General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases.

ABSTRACT: The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance. A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species. The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly. Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly. A limited number of these probes exist in the literature or have been assessed with such pathwa

SUBMITTER: Reid AJ 

PROVIDER: S-EPMC7132332 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature