Dataset Information

16HBE Cell Lipid Mediator Responses to Mono and Co-Infections with Respiratory Pathogens.

ABSTRACT: Respiratory tract infections are a global health problem. The main causative agents of these infections are influenza A virus (IAV), Staphylococcusaureus (S.aureus), and Streptococcuspneumoniae (S.pneumoniae). Major research focuses on genetics and immune responses in these infections. Eicosanoids and other oxylipins are host-derived lipid mediators that play an important role in the activation and resolution of inflammation. In this study, we assess, for the first time, the different intracellular profiles of these bioactive lipid mediators during S.aureus LUG2012, S.pneumoniae TIGR4, IAV, and corresponding viral and bacterial co-infections of 16HBE cells. We observed a multitude of altered lipid mediators. Changes in the amount of 5-hydroxyeicosatetraenoic acid (5-HETE) were prominent for all bacterial infections. The infection with S.pneumoniae showed the strongest impact on bioactive lipid production and led to alterations in the amount of PPARγ ligands and precursors of pro-resolving lipid mediators.

SUBMITTER: Schultz D

PROVIDER: S-EPMC7142531 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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16HBE Cell Lipid Mediator Responses to Mono and Co-Infections with Respiratory Pathogens.

Schultz Daniel D Surabhi Surabhi S Stelling Nicolas N Rothe Michael M Group KoInfekt Study KS Methling Karen K Hammerschmidt Sven S Siemens Nikolai N Lalk Michael M

Metabolites 20200318 3

Respiratory tract infections are a global health problem. The main causative agents of these infections are influenza A virus (IAV), Staphylococcus aureus (S. aureus), and Streptococcus pneumoniae (S. pneumoniae). Major research focuses on genetics and immune responses in these infections. Eicosanoids and other oxylipins are host-derived lipid mediators that play an important role in the activation and resolution of inflammation. In this st ...[more]

PMID: 32197522

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Project description:ObjectiveThis study aimed to assess the prevalence and distribution of respiratory pathogens in children under 18 years old with Acute Respiratory Infections (ARTIs) in Lanzhou, Northwest China, from July 2019 to January 2024.MethodsThe respiratory pathogens studied were FluA, FluB, PIV, RSV, ADV, MP, CP, CB, and LP, detected by indirect immunofluorescence assay (IIF). Data were obtained from the laboratory information system (LIS) of the Lanzhou University Second Hospital. As in Lanzhou, NPIs were implemented in January 2020, and were lifted in December 2022, data were divided into pre-NPIs (July 2019 to December 2019), NPIs (January 2020 to December 2022) and post-NPIs (January 2023 to January 2024) periods for analysis. Pearson's chi-square test, ANOVA, and Fisher's exact test were used to evaluate statistical significance in variable differences, with P < 0.05 considered significant.ResultsA total of 29,659 children diagnosed with ARTIs were included in the study, with 13030(43.93%) test positive for at least one pathogen. Single-pathogen infections predominated (33.10%), while co-detection of MP and PIV was the most common among multi-pathogen cases (52.96%). Pathogen detection rates were notably higher in female children (50.62%) and preschool-aged children (53.45%) and exhibited seasonal variations, with a pronounced increase in winter (47.61%) and a peak in November (48.92%). MP had the highest detection rate (38.59%), followed by PIV (10.18%). Detection rates significantly increased following the lifting of NPIs, rising from 33.82% (SD ± 13.13) during NPIs to 64.42% (SD ± 4.67) (P < 0.001), with 2023 showing the highest detection rate (64.61%) and largest participant count (9,591). In November 2023, detection rates reached their highest level at 73.09%. Post-NPI, most pathogens, except CB and LP, demonstrated significantly higher prevalence (P<0.001).ConclusionIn the Lanzhou region, MP and PIV were identified as the most prevalent respiratory pathogens among children with ARTIs, with peak detection rates during the winter season. Boys and school-age children exhibited higher susceptibility to these infections. NPIs played a critical role in reducing respiratory pathogen transmission. Once NPIs were lifted, a marked resurgence in pathogen incidence highlighted their impact on controlling infection spread.

Dataset Information

16HBE Cell Lipid Mediator Responses to Mono and Co-Infections with Respiratory Pathogens.

Publications

16HBE Cell Lipid Mediator Responses to Mono and Co-Infections with Respiratory Pathogens.

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