Cerebrospinal fluid lipidomics: effects of an intravenous triglyceride infusion and apoE status.

ABSTRACT:

Introduction

High-fat diets increase risk for Alzheimer's disease, but individuals with the risk gene APOE ε4 (E4) paradoxically have improved memory soon after high fat feeding. Little is known about how dietary lipids affect CNS lipids, especially in older adults.

Objectives

We analyzed the lipidomic signature of cerebrospinal fluid (CSF) in older adults who underwent both a saline and TG infusion. We further analyzed these data by E4 carrier status.

Methods

Older adults (n = 21, age 67.7 ± 8.6) underwent a 5-h TG and saline infusion on different days in random crossover design; lumbar CSF was collected at the end of the infusion. Lipids were extracted using dichloromethane/methanol and 13 classes of lipids analyzed using the Lipidyzer platform consisting of an AB S

SUBMITTER: Hanson AJ 

PROVIDER: S-EPMC7147960 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature