Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H⁺ pumps.

ABSTRACT: Schizorhodopsins (SzRs), a rhodopsin family first identified in Asgard archaea, the archaeal group closest to eukaryotes, are present at a phylogenetically intermediate position between typical microbial rhodopsins and heliorhodopsins. However, the biological function and molecular properties of SzRs have not been reported. Here, SzRs from Asgardarchaeota and from a yet unknown microorganism are expressed in Escherichia coli and mammalian cells, and ion transport assays and patch clamp analyses are used to demonstrate SzR as a novel type of light-driven inward H⁺ pump. The mutation of a cytoplasmic glutamate inhibited inward H⁺ transport, suggesting that it functions as a cytoplasmic H⁺ acceptor. The function, trimeric structure, and H⁺ tr

SUBMITTER: Inoue K 

PROVIDER: S-EPMC7148096 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature