Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones.

ABSTRACT: Mediator of cell motility 1 (MEMO1) is a ubiquitously expressed modulator of cellular responses to growth factors including FGF23 signaling, and Memo1-deficient mice share some phenotypic traits with Fgf23- or Klotho-deficient mouse models. Here, we tested whether Memo1 gene expression is regulated by calciotropic hormones or by changing the dietary mineral load. MLO-Y4 osteocyte-like cells were cultured and treated with 1,25(OH)₂ -vitamin D₃ . Wild-type C57BL/6N mice underwent treatments with 1,25(OH)₂ -vitamin D₃ , parathyroid hormone, 17β-estradiol or vehicle. Other cohorts of C57BL/6N mice were fed diets varying in calcium or phosphate content. Expression of Memo1 and control genes was assessed by qPCR. 1,25(OH)₂ -vitamin D_3</

SUBMITTER: Moor MB 

PROVIDER: S-EPMC7156332 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature