Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.

ABSTRACT: Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.

SUBMITTER: Wu J 

PROVIDER: S-EPMC7199528 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature