The histone H4 basic patch regulates SAGA-mediated H2B deubiquitination and histone acetylation.

ABSTRACT: Histone H2B monoubiquitylation (H2Bub1) has central functions in multiple DNA-templated processes, including gene transcription, DNA repair, and replication. H2Bub1 also is required for the trans-histone regulation of H3K4 and H3K79 methylation. Although previous studies have elucidated the basic mechanisms that establish and remove H2Bub1, we have only an incomplete understanding of how H2Bub1 is regulated. We report here that the histone H4 basic patch regulates H2Bub1. Yeast cells with arginine-to-alanine mutations in the H4 basic patch (H4_2RA) exhibited a significant loss of global H2Bub1. H4_2RA mutant yeast strains also displayed chemotoxin sensitivities similar to, but less severe than, strains containing a complete loss of H2Bub1. We found that the H4 ba

SUBMITTER: Meriesh HA 

PROVIDER: S-EPMC7212633 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature