Label-free multiphoton imaging allows brain tumor recognition based on texture analysis-a study of 382 tumor patients.

ABSTRACT:

Background

Label-free multiphoton microscopy has been suggested for intraoperative recognition and delineation of brain tumors. For any future clinical application, appropriate approaches for image acquisition and analysis have to be developed. Moreover, an evaluation of the reliability of the approach, taking into account inter- and intrapatient variability, is needed.

Methods

Coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence (TPEF), and second-harmonic generation were acquired on cryosections of brain tumors of 382 patients and 28 human nontumor brain samples. Texture parameters of those images were calculated and used as input for linear discriminant analysis.

Results

The combined analysis of texture parameters of the CARS and TPEF signa

SUBMITTER: Uckermann O 

PROVIDER: S-EPMC7212881 | biostudies-literature | 2020 Jan-Dec

REPOSITORIES: biostudies-literature