Plasma-Driven in Situ Production of Hydrogen Peroxide for Biocatalysis.

ABSTRACT: Peroxidases and peroxygenases are promising classes of enzymes for biocatalysis because of their ability to carry out one-electron oxidation reactions and stereoselective oxyfunctionalizations. However, industrial application is limited, as the major drawback is the sensitivity toward the required peroxide substrates. Herein, we report a novel biocatalysis approach to circumvent this shortcoming: in situ production of H₂ O₂ by dielectric barrier discharge plasma. The discharge plasma can be controlled to produce hydrogen peroxide at desired rates, yielding desired concentrations. Using horseradish peroxidase, it is demonstrated that hydrogen peroxide produced by plasma treatment can drive the enzymatic oxidation of model substrates. Fungal peroxygenase is then employe

SUBMITTER: Yayci A 

PROVIDER: S-EPMC7216967 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature