The Analysis of the Anti-Tumor Mechanism of Ursolic Acid Using Connectively Map Approach in Breast Cancer Cells Line MCF-7.

ABSTRACT:

Background

Ursolic acid (UA), a primary bioactive triterpenoid, was reported as an anti-cancer agent. However, the current knowledge of UA and its potential anti-cancer mechanisms and targets in breast cancer cells are limited. In this study, we aimed to illustrate the potential mechanisms and targets of UA in breast cancer cells MCF-7.

Methods

The effect of UA on cell growth was determined in MCF-7 cells by MTT assay. The anti-tumor mechanism of UA was evaluated by microarray, CAMP, and Western blot. Moreover, the molecular docking between UA and potential receptors were predicted by iGEMDOCK software.

Results

The result of MTT assay demonstrated that UA could inhibit MCF-7 cell growth with IC₅₀ values of 20 μM. Microarray and CMAP analysis, validated by W

SUBMITTER: Guo W 

PROVIDER: S-EPMC7237111 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature