Glycolaldehyde is an endogenous source of lysine N-pyrrolation.

ABSTRACT: Lysine N-pyrrolation converts lysine residues to N^ϵ-pyrrole-l-lysine (pyrK) in a covalent modification reaction that significantly affects the chemical properties of proteins, causing them to mimic DNA. pyrK in proteins has been detected in vivo, indicating that pyrrolation occurs as an endogenous reaction. However, the source of pyrK remains unknown. In this study, on the basis of our observation in vitro that pyrK is present in oxidized low-density lipoprotein and in modified proteins with oxidized polyunsaturated fatty acids, we used LC-electrospray ionization-MS/MS coupled with a stable isotope dilution method to perform activity-guided separation of active molecules in oxidized lipids and identified glycolaldehyde (GA) as a pyrK source. The resul

SUBMITTER: Chikazawa M 

PROVIDER: S-EPMC7261792 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature