Lamina-Dependent Stretching and Unconventional Chromosome Compartments in Early C. elegans Embryos.

ABSTRACT: Current models suggest that chromosome domains segregate into either an active (A) or inactive (B) compartment. B-compartment chromatin is physically separated from the A compartment and compacted by the nuclear lamina. To examine these models in the developmental context of C. elegans embryogenesis, we undertook chromosome tracing to map the trajectories of entire autosomes. Early embryonic chromosomes organized into an unconventional barbell-like configuration, with two densely folded B compartments separated by a central A compartment. Upon gastrulation, this conformation matured into conventional A/B compartments. We used unsupervised clustering to uncover subpopulations with differing folding properties and variable positioning of compartment boundaries. These conformations relied on

SUBMITTER: Sawh AN 

PROVIDER: S-EPMC7263362 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature