Initiator and executioner caspases in salivary gland apoptosis of Rhipicephalus haemaphysaloides.

ABSTRACT:

Background

Apoptosis is fundamental in maintaining cell balance in multicellular organisms, and caspases play a crucial role in apoptosis pathways. It is reported that apoptosis plays an important role in tick salivary gland degeneration. Several different caspases have been found in ticks, but the interactions between them are currently unknown. Here, we report three new caspases, isolated from the salivary glands of the tick Rhipicephalus haemaphysaloides.

Methods

The full-length cDNA of the RhCaspases 7, 8 and 9 genes were obtained by transcriptome, and RhCaspases 7, 8 and 9 were expressed in E. coli; after protein purification and immunization in mice, specific polyclonal antibodies (PcAb) were created in response to the recombinant protein. Reverse-transcription quantit

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7275347 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature