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Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2.

ABSTRACT:

Background

Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid.

Methods

A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection.

Results

This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.

SUBMITTER: Lau YL

PROVIDER: S-EPMC7275676 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2.

Lau Yee Ling YL Ismail Ilyiana I Mustapa Nur Izati NI Lai Meng Yee MY Tuan Soh Tuan Suhaila TS Hassan Afifah A Peariasamy Kalaiarasu M KM Lee Yee Leng YL Chong Yoong Min YM Sam I-Ching IC Goh Pik Pin PP

PeerJ 20200603

<h4>Background</h4>Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid.<h4>Methods</h4>A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection.<h4>Results</h4> ...[more]

PMID: 32547882

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