Transcriptome-wide organization of subcellular microenvironments revealed by ATLAS-Seq.

ABSTRACT: Subcellular organization of RNAs and proteins is critical for cell function, but we still lack global maps and conceptual frameworks for how these molecules are localized in cells and tissues. Here, we introduce ATLAS-Seq, which generates transcriptomes and proteomes from detergent-free tissue lysates fractionated across a sucrose gradient. Proteomic analysis of fractions confirmed separation of subcellular compartments. Unexpectedly, RNAs tended to co-sediment with other RNAs in similar protein complexes, cellular compartments, or with similar biological functions. With the exception of those encoding secreted proteins, most RNAs sedimented differently than their encoded protein counterparts. To identify RNA binding proteins potentially driving these patterns, we correlated their sediment

SUBMITTER: Adekunle DA 

PROVIDER: S-EPMC7293051 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature