Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion.

ABSTRACT: Understanding complex tissues requires single-cell deconstruction of gene regulation with precision and scale. Here, we assess the performance of a massively parallel droplet-based method for mapping transposase-accessible chromatin in single cells using sequencing (scATAC-seq). We apply scATAC-seq to obtain chromatin profiles of more than 200,000 single cells in human blood and basal cell carcinoma. In blood, application of scATAC-seq enables marker-free identification of cell type-specific cis- and trans-regulatory elements, mapping of disease-associated enhancer activity and reconstruction of trajectories of cellular differentiation. In basal cell carcinoma, application of scATAC-seq reveals regulatory networks in malignant, stromal and immune cells in the tumor microenvironment. Analys

SUBMITTER: Satpathy AT 

PROVIDER: S-EPMC7299161 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature