Additive Modulation of DNA-DNA Interactions by Interstitial Ions.

ABSTRACT: Quantitative understanding of biomolecular electrostatics, particularly involving multivalent ions and highly charged surfaces, remains lacking. Ion-modulated interactions between nucleic acids provide a model system in which electrostatics plays a dominant role. Using ordered DNA arrays neutralized by spherical cobalt³⁺ hexammine and Mg²⁺ ions, we investigate how the interstitial ions modulate DNA-DNA interactions. Using methods of ion counting, osmotic stress, and x-ray diffraction, we systematically determine thermodynamic quantities, including ion chemical potentials, ion partition, DNA osmotic pressure and force, and DNA-DNA spacing. Analyses of the multidimensional data provide quantitative insights into their interdependencies. The key finding of this study is

SUBMITTER: Meng W 

PROVIDER: S-EPMC7300303 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature