DNA methylation signatures of Prostate Cancer in peripheral T-cells.

ABSTRACT:

Background

Prostate Cancer (PCa) is the second most common cancer in men where advancements have been made for early detection using imaging techniques, however these are limited by lesion size. Immune surveillance has emerged as an effective approach for early detection and to monitor disease progression. In recent studies, we have shown that host peripheral blood immune cells undergo changes in DNA methylation in liver and breast cancer.

Methods

In the current study, we examined the DNA methylation status of peripheral blood T cells of men with positive biopsy for PCa versus men with negative biopsy having benign prostate tissue, defined as controls. T cells DNA was isolated and subjected to Illumina Infinium methylation EPIC array and validated using Illumina amplicon seq

SUBMITTER: Mehdi A 

PROVIDER: S-EPMC7310561 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature