MARES, a replicable pipeline and curated reference database for marine eukaryote metabarcoding.

ABSTRACT: The use of DNA metabarcoding to characterise the biodiversity of environmental and community samples has exploded in recent years. However, taxonomic inferences from these studies are contingent on the quality and completeness of the sequence reference database used to characterise sample species-composition. In response, studies often develop custom reference databases to improve species assignment. The disadvantage of this approach is that it limits the potential for database re-use, and the transferability of inferences across studies. Here, we present the MARine Eukaryote Species (MARES) reference database for use in marine metabarcoding studies, created using a transparent and reproducible pipeline. MARES includes all COI sequences available in GenBank and BOLD for marine taxa, unifie

SUBMITTER: Arranz V 

PROVIDER: S-EPMC7334202 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature