Gateway-compatible vectors for functional analysis of proteins in cell type specific manner.

ABSTRACT:

Background

Genetically encoded fluorescent proteins are often used to label proteins and study protein function and localization in vivo. Traditional cloning methods mediated by restriction digestion and ligation are time-consuming and sometimes difficult due to the lack of suitable restriction sites. Invitrogen developed the Gateway cloning system based on the site-specific DNA recombination, which allows for digestion-free cloning. Most gateway destination vectors available for use in plants employ either the 35S or ubiquitin promoters, which confer high-level, ubiquitous expression. There are far fewer options for moderate, cell-type specific expression.

Results

Here we report on the construction of a Gateway-compatible cloning system (SWU vectors) to rapidly tag various

SUBMITTER: Zhang L 

PROVIDER: S-EPMC7339564 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature