Influence of Blood Collection Methods and Long-Term Plasma Storage on Quorum-Sensing Peptide Stability.

ABSTRACT: Finding adequate biomarkers for rapid and accurate disease detection, prognosis, and therapy is increasingly important. Quorum-sensing peptides are herein a new emerging group, produced by bacteria, fungi, protozoa, and viruses, with blood being the most straightforward sample type to detect/quantitate them. However, detailed information about suitable blood sample collection methods and storage conditions for measuring these quorum-sensing peptides hampers further clinical research and development. Here, we first tested the time-dependent stability of a set of chemically diverse quorum-sensing peptides, spiked in blood at different temperatures (4, 21, and 37 °C) in four different ethylenediamine tetraacetic acid (EDTA)-containing plasma tubes (with different protein-stabilizing additives

SUBMITTER: Debunne N 

PROVIDER: S-EPMC7346264 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature