Pre-Growth Culture Conditions Affect Type 1 Fimbriae-Dependent Adhesion of Salmonella.

ABSTRACT: Among various fimbrial structures used by Salmonella enterica to colonize host tissues, type 1 fimbriae (T1F) are among the most extensively studied. Although some experiments have shown the importance of T1F in the initial stages of Salmonella infection, their exact role in the infection process is not fully known. We suggested that different outcomes of T1F investigations were due to the use of different pre-infection growth conditions for the induction of the T1F. We utilized qPCR, flow cytometry, and a wide range of adhesion assays to investigate Salmonella Choleraesuis and Salmonella Typhimurium adhesion in the context of T1F expression. We demonstrated that T1F expression was highly dependent on the pre-infection growth conditions. These growth conditions yielded T1F+ and T1F- popula

SUBMITTER: Klasa B 

PROVIDER: S-EPMC7352897 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature