An Optimized Chromatin Immunoprecipitation Protocol for Quantification of Protein-DNA Interactions.

ABSTRACT: Transcription factors are important regulators of cell fate and function. Knowledge about where transcription factors are bound in the genome is crucial for understanding their function. A common method to study protein-DNA interactions is chromatin immunoprecipitation (ChIP). Here, we present a revised ChIP protocol to determine protein-DNA interactions for the yeast Saccharomyces cerevisiae. We optimized several aspects of the procedure, including cross-linking and quenching, cell lysis, and immunoprecipitation steps. This protocol facilitates sensitive and reproducible quantitation of protein-DNA interactions. For complete details on the use and execution of this protocol, please refer to (de Jonge et al., 2019).

SUBMITTER: de Jonge WJ 

PROVIDER: S-EPMC7357673 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature