Interference of pH buffer with Pb2+-peripheral domain interactions: obstacle or opportunity?
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ABSTRACT:
SUBMITTER: Katti S
PROVIDER: S-EPMC7357838 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Publications
Interference of pH buffer with Pb<sup>2+</sup>-peripheral domain interactions: obstacle or opportunity?
Katti Sachin S Igumenova Tatyana I TI
Metallomics : integrated biometal science 20200213 2
Pb<sup>2+</sup> is a xenobiotic metal ion that competes for Ca<sup>2+</sup>-binding sites in proteins. Using the peripheral Ca<sup>2+</sup>-sensing domains of Syt1, we show that the chelating pH buffer Bis-Tris enables identification and functional characterization of high-affinity Pb<sup>2+</sup> sites that are likely to be targeted by bioavailable Pb<sup>2+</sup>. ...[more]
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