Scalable High-Performance Production of Recombinant Horseradish Peroxidase from E. coli Inclusion Bodies.

ABSTRACT: Horseradish peroxidase (HRP), an enzyme omnipresent in biotechnology, is still produced from hairy root cultures, although this procedure is time-consuming and only gives low yields. In addition, the plant-derived enzyme preparation consists of a variable mixture of isoenzymes with high batch-to-batch variation preventing its use in therapeutic applications. In this study, we present a novel and scalable recombinant HRP production process in Escherichia coli that yields a highly pure, active and homogeneous single isoenzyme. We successfully developed a multi-step inclusion body process giving a final yield of 960 mg active HRP/L culture medium with a purity of ≥99% determined by size-exclusion high-performance liquid chromatography (SEC-HPLC). The Reinheitszahl, as well as the activity wit

SUBMITTER: Humer D 

PROVIDER: S-EPMC7369975 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature