Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples.

ABSTRACT: While identifying acute HIV infection is critical to providing prompt treatment to HIV-positive individuals and preventing transmission, existing laboratory-based testing methods are too complex to perform at the point of care. Specifically, molecular techniques can detect HIV RNA within 8-10 days of transmission but require laboratory infrastructure for cold-chain reagent storage and extensive sample preparation performed by trained personnel. Here, we demonstrate our point-of-care microfluidic rapid and autonomous analysis device (microRAAD) that automatically detects HIV RNA from whole blood. Inside microRAAD, we incorporate vitrified amplification reagents, thermally-actuated valves for fluidic control, and a temperature control circuit for low-power heating. Reverse transcription loop

SUBMITTER: Phillips EA 

PROVIDER: S-EPMC7384476 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature