Bulk and single-cell gene expression analyses reveal aging human choriocapillaris has pro-inflammatory phenotype.

ABSTRACT: The human choroidal vasculature is subject to age-related structural and gene expression changes implicated in age-related macular degeneration (AMD). In this study, we performed both bulk and single-cell RNA sequencing on infant (n = 4 for bulk experiments, n = 2 for single-cell experiments) and adult (n = 13 for bulk experiments, n = 6 for single-cell experiments) human donors to characterize how choroidal gene expression changes with age. Differential expression analysis revealed that aged choroidal samples were enriched in genes encoding pro-inflammatory transcription factors and leukocyte transendothelial cell migration adhesion proteins. Such genes were observed to be differentially expressed specifically within choroidal endothelial cells at the single-cell level. Immunohistochemist

SUBMITTER: Voigt AP 

PROVIDER: S-EPMC7396301 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature