Identification and Functional Analysis of EPOR⁺ Tumor-Associated Macrophages in Human Osteosarcoma Lung Metastasis.

ABSTRACT:

Background

Tissue-resident macrophages can be educated to tumor-associated macrophages (TAMs) by the tumor microenvironment and many types of macrophages express erythropoietic receptor (EPOR); However, little is known about the expression of EPOR on TAMs and the identity of EPOR⁺ TAMs in osteosarcoma lung metastasis has thus far remained elusive.

Methods

EPOR-eGFPcre mice were used to determine the expression of EPOR on lung tissue-resident macrophages. Flow cytometry, RT-PCR, and Western blot were examined to define the identity of EPOR⁺ TAMs in 106 osteosarcoma lung metastasis specimens. Moreover, the clinicopathologic factors and prognosis of patients with CD163⁺EPOR⁺ macrophages were compared.

Results

We found that a su

SUBMITTER: Li Y 

PROVIDER: S-EPMC7450330 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature