MicroRNA-214-3p inhibits the stem-like properties of lung squamous cell cancer by targeting YAP1.

ABSTRACT:

Background

Emerging evidence reveals that microRNAs (miRNAs) play a crucial role in tumor progression, but the underlying mechanism of microRNAs in lung squamous cell cancer (LSCC) remains unclear.

Method

Western-blotting and quantitative real-time PCR (q-PCR) were carried out to detect mRNA and protein expression. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8), colony-forming assay or sphere-forming assay, respectively.

Results

MiR-214-3p was markedly de-regulated in LSCC tissues and was inversely related to the level of Yes-associated protein1 (YAP1), which is the core transcription regulator of the Hippo signaling pathway. Kaplan-Meier survival curves illustrated that patients with high miR-214-3p expression demonstrated more favorable clinical out

SUBMITTER: Lu T 

PROVIDER: S-EPMC7450582 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature