Insights into the molecular-level effects of atmospheric and room-temperature plasma on mononucleotides and single-stranded homo- and hetero-oligonucleotides.

ABSTRACT: Atmospheric and room-temperature plasma (ARTP) has been successfully developed as a useful mutation tool for mutation breeding of various microbes and plants as well animals by genetic alterations. However, understanding of the molecular mechanisms underlying the biological responses to ARTP irradiation is still limited. Therefore, to gain a molecular understanding of how irradiation with ARTP damages DNA, we irradiated the artificially synthesized mononucleotides of dATP, dTTP, dGTP, and dCTP, and the oligonucleotides of dA₈, dT₈, dG₈, dC₈, and dA₂dT₂dG₂dC₂ as chemical building blocks of DNA with ARTP for 1-4 min, identified the mononucleotide products using ³¹P- and ¹H-nuclear ma

SUBMITTER: Wang L 

PROVIDER: S-EPMC7459345 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature