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Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant.


ABSTRACT: Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%-50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild-type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8-92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity.

SUBMITTER: Mlcochova P 

PROVIDER: S-EPMC7462534 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant.

Mlcochova Petra P   Collier Dami D   Ritchie Allyson A   Assennato Sonny M SM   Hosmillo Myra M   Goel Neha N   Meng Bo B   Chatterjee Krishna K   Mendoza Vivien V   Temperton Nigel N   Kiss Leo L   James Leo C LC   Ciazynska Katarzyna A KA   Xiong Xiaoli X   Briggs John A G JAG   Nathan James A JA   Mescia Federica F   Bergamaschi Laura L   Zhang Hongyi H   Barmpounakis Petros P   Demeris Nikos N   Skells Richard R   Lyons Paul A PA   Bradley John J   Baker Steven S   Allain Jean Pierre JP   Smith Kenneth G C KGC   Bousfield Rachel R   Wilson Michael M   Sparkes Dominic D   Amoroso Glenn G   Gkrania-Klotsas Effrosyni E   Hardwick Susie S   Boyle Adrian A   Goodfellow Ian I   Gupta Ravindra K RK  

Cell reports. Medicine 20200901 6


Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%-50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to  ...[more]

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