Chicken Ovotransferrin Variants OTF^B and OTF^C Harboring Substitution of GAT (Asp) to AAT (Asn) in the Codon 500 and their Antimicrobial Activity.

ABSTRACT: Chicken egg white ovotransferrin (OTF) has been reported to exist in three electrophoretic variants (OTF^A, OTF^B and OTF^C). In this report, we identified a causal polymorphism between the OTF^B and OTF^C variants in Japanese and Taiwanese native chickens and compared the antibacterial activity between these two variants. The cDNA sequence analyses from Satsumadori oviducts revealed that three non-synonymous SNPs T1809G (Ser52Ala), A2258G (Ile96Val) and G7823A (Asp500Asn) corresponded to the OTF electrophoretic phenotypes. Of the three SNPs, the G7823A mutation perfectly corresponded to the electrophoretic phenotypes OTF^B (G/G, Asp500Asp), OTF^B/C (G/A, Asp500Asn) and OTF^C (A/A, Asn500Asn) in three chicken p

SUBMITTER: Kinoshita K 

PROVIDER: S-EPMC7477163 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature