Lipid composition dictates serum stability of reconstituted high-density lipoproteins: implications for in vivo applications.

ABSTRACT: Nanolipoprotein particles (NLPs) are reconstituted high-density lipoproteins, consisting of a phospholipid bilayer stabilized by an apolipoprotein scaffold protein. This class of nanoparticle has been a vital tool in the study of membrane proteins, and in recent years has been increasingly used for in vivo applications. Previous work demonstrated that the composition of the lipid bilayer component affects the stability of these particles in serum solutions. In the current study, NLPs assembled with phosphatidylcholine lipids featuring different acyl chain structures were systematically tested to understand the effect that lipid composition has on NLP stability in both neat serum and cell culture media supplemented with 10% serum by volume. The time at which 50% of the particles dissociate,

SUBMITTER: Gilmore SF 

PROVIDER: S-EPMC7485573 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature