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An Optimized Protocol for ChIP-Seq from Human Embryonic Stem Cell Cultures.

ABSTRACT: Chromatin immunoprecipitation (ChIP) followed by next-generation sequencing is a powerful technique that characterizes the genome-wide DNA-binding profile of a protein of interest. The general ChIP-seq workflow has been applied widely to many sample types and target proteins, but sample-specific optimization of various steps is necessary to achieve high-quality data. This protocol is specifically optimized for cultured human embryonic stem cells (hESCs), including steps to check sample quality and non-specific enrichment of "hyper-ChIPable" regions prior to sequencing. For complete details on the use and execution of this protocol, please refer to Gunne-Braden et al. (2020).

SUBMITTER: Sullivan AE

PROVIDER: S-EPMC7501726 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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An Optimized Protocol for ChIP-Seq from Human Embryonic Stem Cell Cultures.

Sullivan Adrienne E AE Santos Silvia D M SDM

STAR protocols 20200918 2

Chromatin immunoprecipitation (ChIP) followed by next-generation sequencing is a powerful technique that characterizes the genome-wide DNA-binding profile of a protein of interest. The general ChIP-seq workflow has been applied widely to many sample types and target proteins, but sample-specific optimization of various steps is necessary to achieve high-quality data. This protocol is specifically optimized for cultured human embryonic stem cells (hESCs), including steps to check sample quality a ...[more]

PMID: 33000002

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