Lacrimal Gland Myoepithelial Cells Are Altered in a Mouse Model of Dry Eye Disease.

ABSTRACT: The purpose of this study was to determine the pathogenic changes that occur in myoepithelial cells (MECs) from lacrimal glands of a mouse model of Sjögren syndrome. MECs were cultured from lacrimal glands of C57BL/6J [wild type (WT)] and thrombospondin 1 null (TSP1^-/-, alias Thbs1^-/-) mice and from mice expressing α-smooth muscle actin-green fluorescent protein that labels MECs. MECs were stimulated with cholinergic and α₁-adrenergic agonists, vasoactive intestinal peptide (VIP), and the purinergic agonists ATP and UTP. Then intracellular [Ca²⁺] was measured using fura-2, and contraction was observed using live cell imaging. Expression of purinergic receptors was determined by Western blot analysis, and mRNA expression was analyzed by microarray

SUBMITTER: Garcia-Posadas L 

PROVIDER: S-EPMC7520664 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature