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Computational Design of a Novel VLP-Based Vaccine for Hepatitis B Virus.

ABSTRACT: Hepatitis B virus (HBV) is a global virus responsible for a universal disease burden for millions of people. Various vaccination strategies have been developed using viral vector, nucleic acid, protein, peptide, and virus-like particles (VLPs) to stimulate favorable immune responses against HBV. Given the pivotal role of specific immune responses of hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) in infection control, we designed a VLP-based vaccine by placing the antibody-binding fragments of HBsAg in the major immunodominant region (MIR) epitope of HBcAg to stimulate multilateral immunity. A computational approach was employed to predict and evaluate the conservation, antigenicity, allergenicity, and immunogenicity of the construct. Modeling and molecular dynamics (MD) demonstrated the folding stability of HBcAg as a carrier in inserting Myrcludex and "a" determinant of HBsAg. Regions 1-50 and 118-150 of HBsAg were considered to have the highest stability to be involved in the designed vaccine. Molecular docking revealed appropriate interactions between the B cell epitope of the designed vaccine and the antibodies. Totally, the final construct was promising for inducing humoral and cellular responses against HBV.

SUBMITTER: Mobini S

PROVIDER: S-EPMC7521014 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Computational Design of a Novel VLP-Based Vaccine for Hepatitis B Virus.

Mobini Saeed S Chizari Milad M Mafakher Ladan L Rismani Elmira E Rismani Elham E

Frontiers in immunology 20200827

Hepatitis B virus (HBV) is a global virus responsible for a universal disease burden for millions of people. Various vaccination strategies have been developed using viral vector, nucleic acid, protein, peptide, and virus-like particles (VLPs) to stimulate favorable immune responses against HBV. Given the pivotal role of specific immune responses of hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) in infection control, we designed a VLP-based vaccine by placing the antibo ...[more]

PMID: 33042118

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Computational Design of a Novel VLP-Based Vaccine for Hepatitis B Virus.

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Computational Design of a Novel VLP-Based Vaccine for Hepatitis B Virus.

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