Following the community development of SIHUMIx - a new intestinal in vitro model for bioreactor use.

ABSTRACT: Diverse intestinal microbiota is frequently used in in vitro bioreactor models to study the effects of diet, chemical contaminations, or medication. However, the reproducible cultivation of fecal microbiota is challenging and the resultant communities behave highly dynamic. To approach the issue of reproducibility in in vitro models, we established an intestinal microbiota model community of reduced complexity, SIHUMIx, as a valuable model for in vitro use. The development of the SIHUMIx community was monitored over time with methods covering the cellular and the molecular level. We used microbial flow cytometry, intact protein profiling and terminal restriction fragment length polymorphism analysis to assess community structure. In parallel, we analyzed the functional

SUBMITTER: Krause JL 

PROVIDER: S-EPMC7524388 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature